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The merged and single-stained images for four representative multiplex immunofluorescence (mIF) of tumor microenvironment (TME). Typical mIF images of a patient receiving sintilimab plus chemotherapy ( A ) and placebo plus chemotherapy ( D ). Immune cell infiltration levels in the tumor tissue before sintilimab plus chemotherapy ( B ) and placebo plus chemotherapy ( C ) between patients in the well-responder group and those in the poor-responder group assessed by multiplex immunofluorescence (mIF). Scale bar:100 μm

Journal: BMC Cancer

Article Title: The immune signatures predict gastric/gastroesophageal junction cancer response to first-line anti-PD-1 blockade or chemotherapy

doi: 10.1186/s12885-025-14805-6

Figure Lengend Snippet: The merged and single-stained images for four representative multiplex immunofluorescence (mIF) of tumor microenvironment (TME). Typical mIF images of a patient receiving sintilimab plus chemotherapy ( A ) and placebo plus chemotherapy ( D ). Immune cell infiltration levels in the tumor tissue before sintilimab plus chemotherapy ( B ) and placebo plus chemotherapy ( C ) between patients in the well-responder group and those in the poor-responder group assessed by multiplex immunofluorescence (mIF). Scale bar:100 μm

Article Snippet: The immunofluorescence slides were scanned using the Vectra 3.0 Automated Quantitative Pathology Imaging System (Akoya Biosciences) equipped with a 20 × objective and visualized using Phenochart software.

Techniques: Staining, Multiplex Assay, Immunofluorescence